DISPOSABLE COUNTING CHAMBERS C-Chip INCYTO
Disposable plastic hemocytometer that presents a significant improvement over conventional glass hemocytometers.
Ensures reliability and repeatability of results through comprehensive quality control.
Eliminates the need for washing.
Reduces exposure to infectious samples and contamination.
Being made of high-strength plastic, it is not brittle.
Neubauer, Improved, Fusch Rosenthal, Burker, Semen, Thoma, Malassez, Petroff Hauser, No grid for CASA, are the models that we have.
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DHC-N01 (Neubauer Improved)
DHC-N01 (Neubauer Improved)
The C-Chip disposable hemocytometer is used for manual cell counting. In research settings, it helps to determine cell concentration prior to cell passage, or to assess cell viability after drug treatment; cell counting solution is essential. Obtaining accurate cell counts can be critical when seeding cells for propagation and experimentation.
The accuracy of manual counts with a hemocytometer depends on:
Precise mixing of the sample (no bubbles)
Number of cells counted (practical: 200-500 per 0.1 mm3)
In a conventional glass hemocytometer, improper setting of the chamber and coverslip changes the volume of the sample introduced into the chamber. The C-Chip chamber with integrated coverslip solves the problem. It is good standard practice to use a disposable hemocytometer for clinical laboratories and laboratories that work with infectious materials. It is safer.
The DHC-N01 disposable hemocytometer, C-Chip, has two precision engineered individual counting chambers (100mm deep) with an integrated coverslip. The chamber has ports for loading samples. The Neubauer Improved (NI) grid pattern is embedded in each chamber. Each grid pattern has nine large squares, each measuring 1 x 1 mm and a total counting area of 3 x 3 mm.
APPLICATIONS
Mammalian cell
Blood test (hematology): blood cell count
· Cell culture: measurement of cell concentration, cell viability.
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External size: Width 25mm x length 75mm x thickness 1.6mm
Chamber Depth: 0.1mm
Chamber volume: 10 μl
CALCULATION
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The entire grid in a hemocytometer contains 9 squares, each of which is 1 mm squared. Large cells are counted using the four large corner squares (and the center). If you are using a small cell dense suspension, use the four 1/25-inch square corners plus the middle square in the center square.
The grid consists of 9 large squares, each measuring 1 x 1 mm, and a chamber depth of 0.1 mm. Each square has a total volume of 0.1 mm3 or 10-4cm3. The center square is divided into 25 small squares with triple lines and four corner squares divided into 16 small squares.
Each large square has a surface area of 1.0 mm2, and the depth of the chamber is 0.1 mm. Since there are 1000 mm3 per ml, each large square represents a volume of 0.0001 ml, so it is equal to 1 / 0.0001 ml = 10,000 = (104)
DHC-F01 (Fuchs Rosenthal)
GENERAL INFORMATION
Manual microscopic cell counting in a Fuchs-Rosenthal (FR) chamber has been the gold standard for the quantification of leukocytes in cerebrospinal fluid (CSF). An excellent correlation was observed between the cell counts obtained with the C-Chip camera and the FR camera. ("Determination of leukocyte counts in cerebrospinal fluid with a disposable plastic hemocytometer" Link to http://www.ncbi.nlm.nih.gov/pubmed/17847111 )
C-Chip DHC-F01 eliminates the risk of exposure to potentially infectious materials and the risk of improper chamber and coverglass setting, improving accuracy and repeatability.
C-Chip DHC-F01 eliminates the risk of exposure to potentially infectious materials and the risk of improper fitting of the chamber and the cover slip, which improves the precision and repeatability.
APPLICATIONS
Applications: cell counts in cerebrospinal fluid (CSF), rare cells.
External size: Width 25mm x length 75mm x thickness 1.6mm
Chamber Depth: 0.2mm
Chamber volume: 20 μl
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CALCULATION
The entire grid in a hemocytometer contains 16 squares, each of which is 1 mm square. A dense small cell suspension is 1/4-inch square.
Each large square has a surface area of 1.0 mm2, and the depth of the chamber is 0.2 mm. Since there are 1000 mm3 per ml, each large square represents a volume of 0.0002 ml, so it is equal to 1 / 0.0002 ml = 5,000. The volume factor is 5,000.